Inhibition abilities of Bacillus spp. to Vibrio parahaemolyticus on early mortality syndrome in Thua Thien Hue

Abstract

Datum were collected on 216 samples, with 4 repetitions each 54 which collected in different shrimp culture ponds in Quang Dien and Phu Vang, Thua Thien Hue province and isolated of Bacillus strains: B. subtilis A , B and B. subtilis B. Bsubtilis BS. Under culture conditions for microbial strains Bacillus (37⁰C incubation, pH 5-8, concentration of NaCl 1-4 %), different strains of Bacillus has tested with Vibrio. The experiments were tested with 20 strains of Vibrio, which were isolated from the EMS on shrimp. There are 4 strains of Vibrio (cholera, VK1; alginolitycus,VD2; mimicus, X1; parahaemolyticus, X2),  which were selected for pairs for inhibition of Bacillus. First results conducted the ability of Bacillus resistance to pathogenic Vibrio as (1) best concentration of Bacillus for inhibition of 10⁷ CFU/ml with Vibrio is 10⁵ CFU/ml and obtained 24.77 ± 0.71 mm of anti-dimension; (2) the optimal time and a highest inhibition after 20 h mixture of Bacillus and Vibrio. Simultaneously, with 12 pairs tested in 3 treatments according to Latin square design and the results were showed two pairs (A - X2) and (A - X1) with maximum anti-dimension of the inhibition to Vibrio, as 35.77 ± 0.83 and 29.83 ± 0.45 mm, respectively. The results and innovation can be announced to farmers and shrimp culture ponds to apply for the prevention of EMS in the region and country.