Designing primers for Nested-MSP amplying RASSF1A (Ras association domain family member 1)

Abstract

Methylation of Tumor Suppressor Genes (TSGs) has been identified as the cause of nasopharyngeal tumorigenesis. Among TSGs, RASSF1A plays an important role in cell cycle regulation, microtubule stablilization, and control ostabilization invasion and metastasis. The methylation occurs on the gene of RASSF1A, which has been reported as the early event of nasopharyngeal carcinoma. Therefore, in this study, the primers for Nested-MSP method will be designed based on bioinformatics tools: Methprimer, IDT, and Annbyb. As the result, primer pairs, including sequence primers, methylation primers, and unmethylation primers, were successfully designed. The length of the PCR assay with these primers were 392bp, 200bp và 173bp, respectively. Thus, this study provides the primer pairs for the Nested-MSP method to further investigate the methylation of RASSF1A gene in nasopharyngeal carcinoma.