Antioxidant activity of isolated compounds from ethyl acetate extract of Bruguiera parviflora

Abstract

Bruguiera parviflora belongs to Bruguiera genus, which possesses several biological activities such as antidiabetic, anti-inflammatory, and antioxidant. Phytochemical investigation of the ethyl acetate extract from Bruguiera parviflora leaves was carried out bioassay-guided isolation and obtained three known compounds, namely kochioside A (1), oleanolic acid (2) and quercitrin (3). The chemical structures of isolated compounds were identified by spectroscopic method and comparison with previously published data. All isolated compounds were evaluated for antioxidant activity with the free radical scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH). Among them, quercitrin (3) was the most potent compound against DPPH radical with the IC₅₀ value of 16.4 ± 0.2 µg/mL, compared to vitamin C, which was used as a positive control.