The optimal protocol for analysis of single nucleotide polymorphism rs320 on Lipoprotein Lipase gene of Vietnamese populations

Abstract

The rs320 polymorphism of LPL gene has been reported to be associated with triglycerides, total cholesterol, HDL-C, and LDL-C. To analyze this polymorphism in Vienamese populations, the study aimed to optimize the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with primers, thermal cycles, and restriction enzyme
which were selected and tested to identify opimal condition of PCR-RFLP method. The GG, TG, and TT genotypes by PCR - RFLP methods were confirmed by the sequencing method. The
experimental results determined the annealing temperature of 57 oC for PCR, the minimum 3 units of HindIII for incubation with PCR product. In conclusion, this optimal PCR-RFLP
method can be applied to genotype LPLrs320 in large populations to investigate the association between the rs320 polymorphism and dyslipidemia.