RAPID IN VITRO PROPAGATION OF NATIVE DENDROBIUM CHRYSOTOXUM

Tóm tắt

The rapid micropropagation of Dendrobium chrysotoxum by applying in vitro tissue culture has the following results: The VW containing 20g/l sucrose and 8g/l agar was the most suitable medium for seed germination. The appropriate medium for rapid protocorm multiplication and shoot formation was the basic MS supplemented with 20g/l sucrose and 8g/l agar. The basic MS containing 20g/l sucrose, 8g/l agar, TDZ 4μM and NAA 1μM was the most optimum medium for shoot propagation from seedlings and gave the best shoot quality. NAA had little effect on generating roots of  Dendrobium chrysotoxum in vitro.