Identification of on some Fungal Strains Parasitic on Coffee Scale Insect by DNA

Abstract

Since 2009 up to date, we collected and isolated 23 strains of mycopathogens from coffee scale insects, among them 8 strains were difficult to identify by morphology. Thus, we applied method of
DNA sequencing and compared with the GeneBank by BLAST nucleotide-nucleotide from the National Center for Biotechnology Information, Bethesda, USA for identification. The results revealed that these 8 strains belong to 6 species as follow: BR2 and BR5 are Beauveria bassiana; MR4 and MR7 are Metarhizium anisopliae; BR12 is Cephalosporium lanoso-niveum; BR7 is Cordyceps nutans; BR15 is Toxicocladosporium sp.; and BR16 is Paecilomyces cicadae.