Selection of Optimal Conditions for Bacillus licheniformis (strain BCRP) Culture to Synthesize Thermostable - amylase

Abstract

In this paper, the results on the selection of optimal culture conditions for Bacillus licheniformis (strain BCRP) to synthesize thermostable α - amylase were presented. The selection of optimal culture conditions (medium, inoculation ratio, culture temperature, pH, culture time...) was performed experimentally. α -  amylsase activity was determined by spectrophotometer. Protein concentration was determined by Bradford method. Culture medium with 3% rice powder instead of soluble starch, inoculation ratio of 3%, medium pH of 7.5, culture at 37^oC and culture time of 42 hours  were selected for culturing B.licheniformis (strain BCRP). The culture schema for B.licheniformis (strain BCRP)  was presented.