Research on Micropropgagation of Aloe vera L. by In vitro Method

Abstract

Aloe Vera  L. is medicinal as well as cosmetic plant.  An experiment was conducted to
investigate the possibility for rapid micropropagation of  Aloe vera for raw material production.
Harvest of explants during spring season and sterilization in 0.1% HgCl2   for 7 minutes resulted in
high sterilization effect for  in vitro initiation. MS medium added with 2.5 ppm BAP gave high
multiplication rate and high quality shoots. Adding 1.5 - 2.0 g/l of active charcoal in rooting medium
provided high rooting effect  and  good root quality. Transplanting  in vitro Aloe vera plantlets into
sand yielded high survival rate and  good plantlet growth. The cropping season had no visible
effect on transplanting in vitro Aloe vera.