Isolation and biological examination of diterpenic-γ-lactones from the leaves of Andrographis paniculata Nees

Abstract

By means of column chromatography four diterpenic γ-lactones were isolated from the leaves of Andrographis paniculata Nees (family Acanthaceae). Their chemical structures were elucidated by means of the modern physical methods (IR, MS and NMR) and identified as andrographolide (DM6) and neoandrographolide (DM7), and a mixture (DM4) consisting of 14-deoxyandrographolide (DA) and 14-deoxy-11,12-didehidroandrographolide (DDA). The preliminary examination of biological activities reveals that both compounds. andrographolide and neoandrographolide inhibit slightly only the strain  Pseudomonas aeruginosa. Besides, andrographolide exhibits the ability to inhibit considerably the growth of Hep-2.