Research on the effects of growth regulators and organic compounds on the in vitro propagation of Dendrobium anosmum Lindl

Abstract

     This study aims to determine the appropriate growth regulators for the rapid shoot multiplication and rooting of Dendrobium anosmum Lindl. in vitro. The results after 45 days showed that growth regulators significantly affected the rapid shoot multiplication and rooting of Dendrobium anosmum Lindl. cultured in vitro. Specifically, the MS medium supplemented with 1 mg/L BA was the most suitable for rapid shoot multiplication, achieving 5.8 shoots. The medium supplemented with 20 % coconut water was the most suitable for plant growth, as indicated by shoot number, leaf number, and shoot height of 2.4 shoots, 3.11 leaves, and 3.44 cm, respectively. The best rooting was observed in the MS medium supplemented with 0.5 mg/L IBA, achieving 3.18 roots. The results of this study contribute to the development of an in vitro micropropagation protocol for Dendrobium anosmum Lindl. to meet the market demand for orchid seedlings.