Establishing technical specifications for softgel capsules containing nanocurcumin

Abstract

   The aim of this research was to development of technical specification of softgel containing curcumin nanoliposom. Curcumin nanoliposom contains Curcuminoid 15.0 mg in form of nanoliposome and recipients (White bee wax, palm oil, lecithin, soybean oil). The procedure firstly isolate demethoxy curcumin, bisdemethoxy curcumin from Curcuma longa powder extract by column chromatography. The mobile phase is CHCl3-MeOH with mobile phase having increasing polarity. Determine characteristics of isolated demethoxy curcumin, bisdemethoxy curcumin: color, solubility, purity (Thin layer chromatpgraphy, Differential Scanning Calorimetry and HPLC), structures (IR, MS, 1H-NMR and 13C-NMR). Using the two curcumin isolated and curcumin (Chromadex, USA) to validate the simutaneous determination of Curcumin, Demethoxy curcumin, Bisdemethoxy curcumin by RP-HPLC method with PDA detector. Establish technical specifications for soft capsules containing nanocurcumin liposome including form, uniformity of weight, solubility test, partical size, identification and assay.