Survey of antioxidant activity and total phenolic content in polygonum cuspidatum polygonaceae

Abstract

   For ages, Polygonum cuspidatum Polygonaceae has been used to treat rheumatoid arthritis, bleeding from injuries. That is why amenorrhea is also a medicine to stop bleeding. Nowadays, one of the development directions is to demonstrate the effect of total extract, fractional and pure active ingredients of this medicinal material with experimental models by modern equipment for scientific research conclusions.Therefore, we carried out the thesis "investigating antioxidant activity and total phenolic content of extract from Polygonum cuspidatum Polygonaceae " with the targets: - Survey of antioxidant effects of total extract and fractional - Building a process for quantifying the total phenolic content of total extract with antioxidant effectResult:The 70% ethanol extraction‟s antioxidant is similar to 16,82% of ascorbic acid. I 50 value of 70% ethanol extraction is 30,67μg/ml. IC50 value of ascorbic acid is 5,16μg/ml.UV/Vis spectrophotometric method for analysis of total phenolic content . The process is tested: Specificity, Linearity (ŷ = 0,0141x + 0,0267, R2 = 1,00 with concentrations around 10,0 - 60,0 (μg/ml)), Repeatability (RSD = 2,7%) and Accuracy (overall 98,86%). Polyphenol content (P%) in the extract (converted into gallic acid): 10,09%.