Isolation, identification and investigation of antibacterial activity of biomass crude extract from Pycnoporus fungi

Abstract

Genus Pycnoporus has been known to have antimicrobial activities against numerous pathogenic bacteria, but so far there have been no in-depth studies to exploit the antibacterial compounds of these fungi in Vietnam. This study selected and successfully isolated one fungus sample that belongs to genus Pycnoporus in Vietnam. The fungi sample was identified as Pycnoporus sanguineus and was successfully cultured in laboratory in solid substrate of rice bran and sawdust. The fungal biomass was extracted with methanol for crude extract and then its antibacterial activity was tested via disc diffusion method. The Pycnoporus sanguineus crude extract at 150 mg showed antibacterial activity against 6 strains of bacteria, including Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli and Vibrio parahaemolyticus. This result opens a premise for further in-depth research on the antimicrobial active compounds from fungi in the future.