Evaluation of rapid detection of Porcine reproductive and respiratory syndrome virus (PRRSV) using Polymerase spiral reaction

Abstract

     Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS viruses (PRRSV) is one of the most complicated and dangerous diseases in pigs with a high mortality rate. In many provinces in Vietnam, annual PRRS outbreaks continue to threaten farmers' livelihoods and consumers' health. RT-PCR is currently the gold standard for diagnosing PRRS, with other molecular biology tools constantly being developed. The isothermal spiral amplification (Polymerase Spiral Reaction - PSR) method was developed and optimized in this study for rapid and specific detection of PRRSV. The method is simple and does not require expensive thermal cycling equipment. The optimized PSR could detect PRRSV target sequence with a detection limit of 10^0.5 TCID50/mL and the reaction was carried out at a constant temperature (65 ⁰C) for 40 minutes. Simultaneously, the diagnostic results could be immediately confirmed by the color of the reaction.