PREPARATION AND CHARACTERIZATION OF FERROUS SULFATE LIPOSOME FORMULATION: EFFECT OF IRON-PHOSPHOLIPID RATIO

Abstract

In this study, three ferrous sulfate-loaded liposome formulations based on the weight ratio of ferrous sulfate to phospholipid including 0.02 (Lipofe 01),
0.04 (Lipofe 02), 0.06 (Lipofe 03) were prepared to evaluate the encapsulation efficiency of liposome, average particle size, polydispersity index (PDI), and zeta
potential. The morphology of the liposomes was determined using TEM measurements. Ferrous sulfate liposomes were prepared through a simple technique,
using glycerol as the lipid solvent and deionized water as the hydration medium. The particle size was reduced with the aid of an ultrasonic probe. The results
showed that the two formulas Lipofe 01 and Lipofe 02 gave acceptable liposome performance and physicochemical properties. Of which, Lipofe 01 gave liposome
encapsulation efficiency, average particle size, PDI, and zeta potential of 65.75%, 135.6nm, 0.35, and -62.15mV, respectively and sample Lipofe 02 gave 56.66%,
153.4nm, 0.43 and -42.07mV, respectively. TEM images show that iron liposomes are oval and elliptical vesicles distributed quite uniformly. Thus, these initial
results show the potential for the preparation of iron sulfate formulation using simple techniques and food-grade solvents for application in the production of
iron supplements.