DETERMINATION OF VITAMIN B6 IN BANANA VINEGAR BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Abstract

Research has been done to quantify the water-soluble vitamin B6, a vitamin
found in banana vinegra. The analysis method of vitamin B6 by HPLC is
established by the following conditions: Column: ZORBAX XDB-C18; Detector:
UV–DAD (210nm); Sample injection volume: 30μL; Mobile phase:
methanol/phosphate buffer solution pH = 2.5 (Sodium 1-hexanesulfonate
0.05M) = 38:62; Flow rate: 0.6mL/min. The calibration curve was experimentally
defned with regression coeffcient R2 = 0.9998 and limit of detection (LOD), limit
of quantification (LOQ) were 0.015, 0.047mg/L, respectively. The method has
been applied to quantify vitamin B6 in order to control the quality of banana
vinegar during the vinegar fermentation.