Effects of Ethyl methane sulfonate on the mutagenesis of gloxinia (Sinningia speciose (G. Lodd.) Hiern) under in vitro conditions

Abstract

This study was to evaluate the effect of Ethyl methane sulfonate on mutant formation in gloxinia (Sinningia speciose (G. Lodd.) Hiern). Stem segments with axillary buds of in vitro gloxinia samples were treated with six different EMS concentrations (0%, 0.2%, 0.4%; 0.6%; 0.8%; 1%) for 1 hour and 2 hours. The results showed that, the higher EMS concentration, longer treatment time, the lower the survival rate, shoot regeneration rate and the more different morphologies appear compared to the control. Among these, EMS concentration of 0.8% for 1 hour and EMS concentration of 0.2% for 2 hours treatments showed a different phenotype of the seedlings compared with the control, and had a high regeneration ability. The ITS regions of these two treatments were sequenced to detect 13 SNPs (Single nucleotide polymorphism), in which EMS treatment at a concentration of 0.8% for 1 hour recorded 12 SNP markers, and 0.2% for 2 hours recorded 1 SNP marker.