STUDY ON FUCOIDAN PURIFICATION FROM Ceratophyllum submersum AGLA

Abstract

Fucoidan is an heterozygous polysaccharide sulfate with complex composition, this compound contains fucose polysaccharide (FCSPs), presented in seaweed, has many interesting biological activities such as anti-cancer, immune, anti inflammation, antiviral, anticoagulant, antioxidant... This study conducted the fucoidan purification from Ceratophyllum submersum (collected in Dien Chau area, Soc Trang province) by Gel chromatography filtration and DEAE-cellulose ion exchange chromatography method. The result of ion exchange chromatography method at retention time of 2 hours, flow rate of 1 mL/minute and salt concentration of 0.5M, the purity of resulting fucoidan achieved (62.05%) was 2.16 times higher than gel filtration chromatography (28.64%).

Keywords: Ceratophyllum submersum, fucoidan, DEAE-cellulose, gel chromatography Sephadex.