PRELIMINARY PURIFICATION OF C-PHYCOCYANIN FROM Ceratophyllum Demersum WITH ACTIVATED CHARCOAL, CHITOSAN AND AMMONIUM SULFATE

Abstract

C-phycocyanin (C-PC) from raw materials - a compound with many important activities (anti-oxidant, anti-inflammatory, anti-cancer) has been extracted and preliminarily clean to increase purity for C-PC. C-PC extracts from the raw materials, after being adsorbed vi activated carbon and chitosan at each survey parameter were precipitated with ammonium sulfate at suitable saturation concentration. The results showed that the concentration of activated carbon and chitosan was 3% (w/v) and 0.3% (v/v) for 15 minutes, ammonium sulfate precipitated to 50% saturation significantly improved the purity of C-PC (2.62) compared with extract (0.40) and recovery efficiency (51.11%).