EFFECT OF DIFFERENT KINDS OF SUGAR ON MULTIPLICATION OF Codonopsis javanica’s ADVENTITIOUS ROOTS IN LIQIUD SHAKE CULTURE

Abstract

In in vitro culture, the addition of sugar to the culture medium is essential, aiming to provide a carbohydrate source for the sample. This study was conducted to investigated the effect of different kinds of sugar on the multiplication of adventitious root biomass of Codonopsis javanica in the liquid shake culture. The results showed that the addition of sugar helped increase the root proliferation coefficient. Appropriate concentrations of four kinds of sugar were 55 g/L fructose, 58 g/L glucose, 14 g/L maltose, 57 g/L sucrose. The growth of adventitious roots on sucrose-supplemented medium was better than fructose, glucose and maltose. After 30 days of incubation, the root proliferation coefficient obtained on medium supplemented with 57 g/L sucrose was 4.16 times. The results of the study are the basis to optimize the process of multiplication of adventitious roots to obtain root biomass of C. javanica.