Study on antioxidant and antifungal abilities of medicinal mushroom Pleurotus tuber-regium

Abstract

This study aims to evaluate the antioxidant and antibacterial capabilities of the medicinal mushroom Pleurotus tuber-regium cultivated in the laboratory. The mushroom fruiting bodies were cultivated, harvested, and crushed, then extracted with 90° ethanol at a ratio of 1:24 (1 gram of mushroom soaked in 24 ml of solvent) for 4 hours and placed in a shaker for another 4 hours, after which the extract was filtered. The obtained extract was concentrated under vacuum to produce the final extract. The antioxidant ability was tested using the DPPH reagent with ascorbic acid (Vitamin C) as the positive control, resulting in inhibition percentage (HTCO %) in mushroom samples increasing from 52.36 % to 92.41 %. And the extract with the highest result has an IC₅₀ = 377.94 µg/ml, while the positive control sample has an IC50 = 79.77 µg/ml. The antibacterial capability was tested using the agar diffusion method on two types of bacteria, B. subtilis and E.coli, with three concentrations of bacteria and three dilution concentrations. The research results show that the Pleurotus tuber-regium mushroom has antibacterial capabilities against both types of bacteria, with the best results against the Gram-positive bacteria B. subtilis, which is better compared to the Gram-negative bacteria E. coli.