Genetic Diversity Analysis of Pyricularia oryzae in the Red River Delta of Viet Nam Using Rep-PCR

Abstract

    Rep-PCR technique (Repetitive sequence primed PCR) with 3 sets of primers designed on the repetitive sequences of bacterial genome including the extragenic repetitive palindromic (REP), enterobacterial repetitive intergenic consensus (ERIC) and BOX element was used to assess the genetic diversity of 22 Pyricularia oryzae isolates isolated from blast infected rice plants collected in 2012 spring season in the Red River Delta of North Viet Nam. PCR using REP primers (REP-PCR) produced polymorphic bands from blast fungus isolates. Rep-PCR analysis showed that blast fungus populations in this region are genetically diverse as previously reported. No clear correlation existed between fugus groups based on Rep-PCR analysis and other characteristics such as the color of the fungal culture, location of collection, and isolates.