Role of Antibiotics on Sperm Motility of Tiger Grouper (Epinephelus fuscoguttatus) after Chilled Storage in Refrigerator

Abstract

      The study was conducted to determine the effect of different antibiotics on sperm motility following chilled storage of Waigieu seaperch Epinephelus fuscoguttatus. Semen were diluted with an extender at a ratio of 1:3 (semen: ASP) in 1.5ml Eppendorf tubes. Experiments were conducted by adding different antibiotics including Neomycin, Gentamycin, or Penicillin plus Streptomycin at concentrations of 200, 400 or 600 ppm. All treatments were refrigerated at 4^oC. The results showed that sperm motility was best when 200 ppm Neomycin was added. Sperms remained motile up to 36 days in this experiment.