Bước đầu khảo sát sự nhiễm Listeria Monocytogenes trong một số thực phẩm trên thị trường Hà Nội bằng kỹ thuật Polymerase chain reaction (PCR)

Tóm tắt

Using PCR techniques developed with LM-F76, LM-R543 primers, the investigation oftheinfection of L. monocytagenes was done with 165 food samples including 116 dairy- and 49meat-based foods. The PCR analysis showed no contamination with L. monocylogenes wasdetected in dairy samples, in contrary, 11149 meat samples was positive with L. monocylogenes. This result was verified by identification of the strains isolated from positive sample on Oxfordagar with 16S rDNA technique. The result confirmed that all strains isolated from PCR positivesamples were identified as L. monocytagenes. This primary analysis gave a first warning to thecontamination risk of L. manocylogenes in food in Hanoi market. It demands a widely investigation for the dead bacterium infection risk in the marketed foods.