DETERMINATION OF NICOTIN IN HUMAN URINE BY USING SOLID-LIQUID EXTRACTION AND GC/MS. PART 2. DETERMINATION OF NICOTINE AND ITS METABOLITES

Abstract

The aim of our research is to determine the content of nicotine in human urine by using solid–liquid extraction technique and gas chromatography- mass pectrometry (GC-MS). In part 1, the procedures of isolation of nicotine from urine by solid–liquid extraction and determination of nicotine by GC-MS were reported. In part 2, the results in determination of nicotine and its metabolite in urine samples taken from 20 non-smoking and 10 smoking volunteers will be reported. As a result, the content of nicotine in urine ranged from 0.042 mg/l to 2.074 mg/l. It is worthy to note that four in ten non-smoking person showed nicotine content higher than

Vietnam’s standard.