Copyright (c) 2025 Journal of Health Sciences
Clinical and paraclinical characteristics and evaluation of surgical outcomes for patients with nasal bone fractures at Hai Phong Medical University Hospital
Background: Prescribing medications for patients with impaired renal function requires appropriate dose adjustments based on estimated glomerular filtration rate (eGFR) to minimize the risk of drug accumulation and related adverse events. However, dose adjustment is often inconsistently applied and lacks systematic implementation in routine clinical practice. Objective: To evaluate the effectiveness of a renal function–based dose adjustment alert tool integrated into the electronic prescribing system at a general hospital. Methods: A pre–post intervention study without a control group was conducted at Hai Phong International General Hospital from January to June 2025. The study included 1,000 inpatient prescriptions containing at least one active ingredient requiring renal dose adjustment based on eGFR, equally divided into pre-intervention (n = 500) and post-intervention (n = 500) groups. Data were processed and analyzed using R software with appropriate statistical tests. Results: There were no statistically significant differences in baseline characteristics (age, sex, length of hospital stay, comorbidities) between the two groups (p > 0.05). The rate of inappropriate dosing prior to intervention ranged from 6.1% to 15.0% depending on the stage of renal impairment, and decreased to between 0% and 5.6% after the intervention, with statistically significant improvements observed in some stages (p < 0.05). The physician acceptance rate of dose adjustment alerts ranged from 75.6% to 91.1%, highest in the anticoagulant and antibiotic groups. Conclusion: Integrating a renal function–based dose adjustment alert tool into the electronic prescribing system significantly reduced dosing errors and was well accepted by clinicians. Further development and long-term clinical outcome evaluations are warranted.
Anti-inflammatory potentials of marine derived fungi from the sea of Co To – Thanh Lan
Objective: This study was conducted to test and identify marine fungal strains with anti-inflammatory activity. Subjects and methods: The study was carried out for 28 strains of marine fungi isolated from the sea of Co To and Thanh Lan, Quang Ninh in 2019. The research utilized experimental methods in biology and chemistry, all experiments were repeated at least three times and the results are expressed as mean ± standard error. Results: 4/28 studied marine fungal strains showed anti-inflammatory activity, especially 3 strains M536, M564, and M613 inhibited NO production triggered by lipopolysaccharide in RAW 264.7 cell line with IC50 value < 20 μg/mL. Identification based on their 18S rRNA gene sequences showed that two strains M536 and M564 belong to the genus Pennicilium and strain M613 belongs to the genus Diplomitoporu. These 3 strains are potential sources of material for further research on anti-inflammatory compounds from marine fungi.
A review of study in pharmacogenetics and pharmacokinetics in Vietnamese population from 2000 to 2022
This literature review study was carried out with the aims of describing pharmacogenetic and pharmacokinetic characteristics in the Vietnamese population from 2000 to 2022. The study has conducted to build the syntax and search action process on pubmed. Results of pharmacogenetic and pharmacokinetic studies in Vietnamese populations with full text written in English. Regarding the pharmacokinetic characteristics, the results of 7 studies were included in the review of drugs such as vancomycin, imipenem, anti-malarial drugs, anti-tuberculosis drugs. NONMEM was a popular method applied to simulate the pharmacokinetic parameters of drugs in Vietnamese people. In addition to describing the pharmacokinetic characteristics of these drugs in the Vietnamese population, the authors of these studies also focused on evaluating special populations such as children and pregnant women. In terms of pharmacogenetics, the results were obtained from 10 studies on CYP2C9, CYP3A, HLA, TPMT, TYMS-TSER, and CYP2D6 genes. PCR was applied to sequence the genes for most of the studies on the Vietnamese population.
Studying The Chemical Composition of Some Medicinal Plants Collected in Cat Hai, Hai Phong
The study was carried out on 7 species of plants collected in the mangrove waters of Cat Hai, Hai Phong, including Cỏ tai hùm (Erigeron canadensis L., Asteraceae), Cốc trắng (Lumnitzera racemosa Willd, Combretaceae), Muống biển (Ipomoea pes-caprae L.), Na biển (Annona glabra L., Annonaceae), Sài hồ nam (Pluchea pteropoda Hemsl., Asteraceae), Rau muối (Chenopodium ficifolium Smith, Amaranthaceae), Vọng đắng (Clerodendrum inerme L., Lamiaceae). The investigated samples were investigated for their chemical composition and polyphenol quantification by specific chemical reactions and Folin-Ciocalteu reagent. The obtained results detected the presence of carotenoids, polyphenols, and tannins in the medicinal herbs. Chromatograms of the total extract and the above-ground fractions of the above medicinal herbs were also recorded, serving as the basis for further chemical composition studies. The polyphenol content of the medicinal herbs Cốc trắng, Cỏ tai hùm, Muống biển, Na biển, Rau muối, Vọng đắng and Sài hồ nam were 33.18, 3.96, 7.04, 16.34, 4.76, 11.32 and 44.29 mg/g, respectively. Two species with high polyphenol content, Sài hồ nam, and Vọng đắng, could be potential sources for research on products to protect human health.
Comparision of the effectiveness of combined therapy sodium-glucose transporter 2 inhibitors versus traditional therapy in treatment of type 2 diabetes with heart failure in Hai Phong International General Hospital
To compare the effectiveness of a combination therapy involving sodium-glucose co-transporter 2 (SGLT2i) inhibitors with traditional therapy in treating type 2 diabetes mellitus (T2DM) accompanied by heart failure, the research team conducted a retrospective study on 106 medical records, including 53 patients treated with traditional therapy and 53 patients treated with combination therapy. This study aimed to achieve two objectives: comparing the effectiveness of the SGLT2i combination therapy against the traditional therapy in treating T2DM with concurrent heart failure. After 3 months of treatment, both patient groups showed a reduction in HbA1C, with the traditional therapy group showing a decrease of 0.71% and the SGLT2i group showing a decrease of 0.72%. This difference was not statistically significant (p > 0.05). Similarly, the percentage of patients achieving the target HbA1C and fasting plasma glucose levels increased in both groups, with the SGLT2i group experiencing a greater increase, although the difference was not statistically significant (p > 0.05). The mean BNP levels also decreased in both groups, with a greater reduction in the SGLT2i group (12.54 pg/mL) compared to the traditional group (7.07 pg/mL); however, this difference was not statistically significant (p > 0.05). Notably, the weight reduction in the SGLT2i group (1.25 kg) was significantly more than in the traditional therapy group (0.44 kg), and this difference was statistically significant (p < 0.05). Overall, the results indicate that the SGLT2i combination therapy offers some benefits over traditional therapy, but the differences in treatment effectiveness between the two groups were not statistically significant for most parameters.
Copyright (c) 2024 Journal of Health Sciences
Developing a list of important drug-drug interactions in inpatient prescription practices at Hai Duong General Hospital 2023
Objective: To develop a list of important drug-drug interactions in inpatient prescription practices at Hai Duong General Hospital. Subjects and Research Methods: The research was conducted in three phases: The first phase involved compiling a theoretical list of drug interactions from the hospital's medication list; The second phase involved identifying drug interactions with a frequency of occurrence ≥ 1% through a survey of 400 inpatient medical records; Finally, a list of drug interactions for clinical practice was compiled by a professional team including physicians and clinical pharmacists. All drug interactions were detected using Micromedex 2.0 software, HDSD, and Decision No. 5948/QD-BYT. Results: Based on the data, the research team developed a list of 25 pairs of contraindicated drug interactions that require attention in inpatient prescription practices. Conclusion: This list of significant drug interactions can be disseminated across departments and integrated into the prescription software to alert users of potential interactions at Hai Duong General Hospital.
Research on botanical characteristics and chemical composition of Selaginella sp. collected in Quang Ninh
Selaginella species have existed for about 400 million years, demonstrating the evolutionary steps of vascular plants. In Vietnam, there are many species of this genus. To provide a basis for accurately determining the scientific name and surveying the chemical composition of a Selaginella species collected in Quang Ninh, the topic was conducted and used methods of describing external morphological characteristics, anatomical characteristics, chemical reactions, and thin layer chromatography. The results described the morphological and anatomical characteristics of the nutritional organs of the research sample and preliminarily concluded the classification position to the genus of this species. In addition, the presence of sterols, fats, glycosides, flavonoids, coumarins, anthranoids, saponins was also indicated. The chromatograms of the total extract and the sample fractions studied with the solvent system n-hexane and ethylacetate (5:1) also obtained good separation results, serving as a basis for further studies on chemical composition.
Drug use in the treatment of gastroduodenal ulcers at Hai Phong Children's Hospital in 2021
Objective: Analyze the current status of drug use in the treatment of gastroduodenal ulcers (PGU) at Hai Phong Children's Hospital in 2021. Method: Retrospective description of medical records of patients with peptic ulcers treated as inpatients at Hai Phong Children's Hospital in 2021. The appropriateness of drug use was assessed based on the Guidelines for the treatment of H.p in children and adolescents of the European Society of Gastroenterology - Hepatology and Nutrition 2016 [1], Guidelines for the diagnosis and treatment of some common diseases in children of the Ministry of Health 2015 [2]. Results: General characteristics, in 313 research records, patients with gastritis (PGU) accounted for the highest rate of 77.0%, the number of patients with Helicobacter pylori (H.p) test results (+) was 81.8%. Among the H.p treatment regimens, the 3-drug regimen was the most common (71.4%), of which the antibiotic Metronidazole was the most used (32.6%). The proton pump inhibitor group Esomeprazole was used in most patients (93.6%). The drug with dosage indications that did not comply with the recommendations was most commonly Tetracycline (24.5%). The most common drug-drug interaction was tetracyclin-Bismuth (66.8%).
Study on the inhibition of alpha-glucosidase and acetylcholinesterase by Stellaria media harvested in Hai Phong
This study was conducted on ”Cỏ sao” (Stellaria media L., Caryophyllaceae) collected in Hai Phong. The chemical composition of the samples was analyzed using specific chemical reactions, thin-layer chromatography (TLC), and the inhibitory effects on alpha-glucosidase and acetylcholinesterase enzymes were tested to evaluate the potential of the herbal material in the treatment of related diseases. The results revealed the presence of carotenoids and cardiac glycosides, while groups such as alkaloids, flavonoids, phytosterols, and saponins were not detected. TLC using a solvent system of n-hexane : ethyl acetate (5:1) showed that the ethyl acetate fraction displayed more distinct and diverse spots than other samples, indicating the presence of various chemical compounds. The herbal samples exhibited little inhibitory activity on the acetylcholinesterase enzyme (IC50 > 256 µg/ml). Similarly, the inhibitory activity on the alpha-glucosidase enzyme was also fragile, with an IC50 value > 256 µg/ml. However, the herbal material still has the potential for further research on other biological properties, such as cell protection and cardiovascular health.
Antibiotic resistance of some gram-negative bacteria at Hai Phong International General Hospital from 2020 to 2024
This study investigates the antibiotic resistance of Gram-negative bacteria at Hai Phong International General Hospital from January 2020 to October 2024. A retrospective analysis was conducted on microbiological test data collected during this period, focusing on the isolation rates and resistance profiles of key bacteria such as Pseudomonas aeruginosa, Acinetobacter baumannii, and Escherichia coli. The results indicated a total of 363 samples analyzed, with Pseudomonas aeruginosa having the highest isolation rate from urine samples (24.24%), followed by sputum (17.36%). Antibiotic resistance analysis revealed resistance rates of 55.56% for Ampicillin-Sulbactam and 50% for Amikacin. Other antibiotics such as Ertapenem (28.82%), Cefepime (28.13%), and Moxifloxacin (30.61%) also exhibited high resistance rates. Acinetobacter baumannii was primarily isolated with a total of 98 samples, where samples from bronchial secretions accounted for 23.47% and urine (17.35%). Doxycycline showed the highest resistance rate (90.63%), followed by Tetracycline (77.14%). Although no resistant strains of Meropenem were found, other carbapenems like Ertapenem and Imipenem exhibited significant resistance rates (50% and 53.13%, respectively). Escherichia coli was isolated positively from 2,436 samples, with an ESBL rate of 26.03%. The majority of Escherichia coli was isolated from urine samples (73.11%). Resistance rates of Escherichia coli to Ciprofloxacin and Moxifloxacin were 50% and 49.65%, respectively. These findings underscore the necessity for continuous monitoring and implementation of antibiotic stewardship programs to address the increasing threat of antibiotic resistance among Gram-negative bacteria in the hospital environment.
Investigation of the Anti-inflammatory Activity of Four Leaf Extracts of Rhodomyrtus tomentosa Collected in Do Son, Hai Phong
This study was conducted on Rhodomyrtus tomentosa (commonly known as Sim), collected from the hilly region of Do Son, Hai Phong, Vietnam. Dried leaf samples of R. tomentosa were subjected to ultrasonic-assisted extraction at room temperature using three solvents: water (RT-N2), ethanol (RT-Et), and methanol (RT-Me), as well as hot water extraction (RT-N1), yielding four different extracts. The anti-inflammatory activity of these extracts was evaluated via the inhibition of nitric oxide (NO) production in RAW 264.7 macrophage cells, in order to assess the therapeutic potential of R. tomentosa in inflammation-related conditions. The results indicated that three extracts RT-Me, RT-Et and RT-N1 are all exhibited moderate to weak anti-inflammatory activity, with IC50 values ranging from 13.53 ± 0.95 µg/mL to 190.75 ± 6.30 µg/mL. In contrast, the RT-N2 extract showed negligible activity compared to the positive control L-NMMA (IC50 = 3.45 ± 0.5 µg/mL). Among the tested extracts, the methanolic extract (RT-Me) demonstrated the most potent inhibitory effect, with an IC50 value of 13.53 ± 0.95 µg/mL. These findings suggest that leaf extracts of Rhodomyrtus tomentosa, particularly the methanolic extract, hold promise for the treatment of inflammatory conditions and may provide a basis for further studies focusing on burn therapy.
Analysis of the current status of antihypertensive drug use at the outpatient hypertention clinic, Van Giang district health center, Hung Yen province, in 2024
Objective: To describe the current situation and analyze the rationality of antihypertensive drug use in patients at the outpatient hypertension clinic of Van Giang District Health Center, Hung Yen Province, in 2024. Methods: A retrospective cross-sectional study was conducted using data from 400 patient medical records at the hypertension clinic from April 1, 2024, to July 31, 2024. Patients included were over 18 years old, had continuous follow-up during the 4-month period, underwent clinical and laboratory monitoring (including mandatory serum creatinine), and had complete blood pressure records throughout the follow-up period. Results: The average age of the study sample was 69,12 ± 9,15 years, with a higher proportion of female patients compared to male patients (52% vs 48%). Among the patients, 89% had hypertension with dyslipidemia, and 61% had angina. Diuretics were the most commonly prescribed drugs (66,69%), followed by angiotensin II receptor blockers (61,69%). A total of 36 different treatment regimens were used; the combination of ARB + diuretic was the most common (38,19%). Most patients achieved target blood pressure, with no significant difference across the 4-month follow-up period. Among hypertensive patients without specific comorbidities, 85,35% received appropriate prescriptions. However, only 16,15% of those with hypertension and angina received appropriate prescriptions. Perindopril was the drug most frequently prescribed inappropriately with respect to renal function, accounting for 41,7% of the 120 prescriptions involving this drug. There were 95 prescriptions with serious drug interactions between ACE inhibitors and ARBs. There are four contraindicated combinations in patients with a creatinine clearance (ClCr) < 30 mL/min: Irbesartan + Hydrochlorothiazide, Telmisartan + Hydrochlorothiazide, Losartan + Hydrochlorothiazide, and Candesartan + Hydrochlorothiazide. Conclusion: The majority of patients at the Hypertension Clinic of Van Giang District Health Center achieved target blood pressure levels; however, physicians’ prescribing practices remain suboptimal in terms of drug selection and dosing, instances of drug interactions and prescriptions given despite contraindications were also observed.
Clinical and paraclinical characteristics and treatment outcomes of mandibular condylar neck fractures at Viet Tiep Hospital in 2024
Objective: To describe the clinical and paraclinical characteristics and evaluate the treatment outcomes of fractures in the mandibular condylar neck region. Methods: A cross-sectional descriptive study was conducted on 32 patients with traumatic fractures in the mandibular condylar neck region, treated at Viet Tiep Friendship Hospital from January 2024 to December 2024. Results: Male patients accounted for 71.9%, with a mean age of 31.3 years. Traffic accidents were the cause in 84.4% of cases. All patients presented with mouth opening limitation and malocclusion. Moderate to severe displacement was observed in 93.7% of cases. After surgery, good occlusion was achieved in 84.4% of patients, and good mouth opening in 90.6%. No cases of infection, facial nerve paralysis, or temporomandibular joint ankylosis were recorded. All patients demonstrated good bone union. Overall, good treatment outcomes were observed in 84.3% of cases. Conclusion: Fractures in the mandibular condylar neck region require accurate diagnosis and timely treatment to ensure proper masticatory function and minimize post-treatment complications.
Metabolic Syndrome in Chronic Kidney Disease Patients on Maintenance Hemodialysis at Kien An Hospital in 2025
Objective: to describe the current status and identify factors associated with metabolic syndrome (MetS) in hemodialysis patients. Methods: A cross-sectional study was conducted on 113 end-stage chronic kidney disease patients undergoing maintenance hemodialysis at the Artificial Kidney Department, Kien An Hospital, Hai Phong, from March to June 2025. Results: Data were collected through direct interviews, anthropometric measurements, and medical records. The results showed a prevalence of MS of 68.1%. The most common components were hypertension (90.9%), low HDL-C (88.3%), and elevated blood glucose (84.4%). Among patients with MetS, 37.7% had 4 components, 36.4% had 3 components, and 26.0% had all 5 components. Logistic regression analysis identified age ≥ 60 (OR=4.20; 95%CI: 1.06–16.68), physical inactivity (OR=2.78; 95%CI: 1.13–6.88), overweight/obesity (OR=4.40; 95%CI: 1.22–15.84), and high waist-to-hip ratio (OR=2.43; 95%CI: 1.02–5.78) as factors increasing the likelihood of metabolic syndrome. Conclusion: This study highlights the heavy burden of metabolic disorders in hemodialysis patients, especially in older adults, those with central obesity, and sedentary lifestyles. Early screening, lifestyle interventions, and comprehensive metabolic control are necessary to improve the quality of life in this patient group.
Current status of online gaming and some factors related to online gaming disorder in students of Tam Nong High School - Phu Tho in 2025
Objective: To determine the rate of online gaming and some factors related to online gaming disorder in students of Tam Nong High School, Phu Tho province in 2025. Method: A cross-sectional study was conducted on 260 students of Tam Nong High School, Phu Tho Province. A self-administered questionnaire was used to survey personal, family, academic, social, and online gaming characteristics. Online gaming disorder was assessed using the VN-IGD-17 scale. Results: The rate of online gaming among students at Tam Nong High School was 89.23%, of which 16.38% of students playing online games had screening scores showing a risk of online gaming disorder, according to the VN-IGD-17 scale. The study identified factors related to students' IGD including: game genre (OR = 2.39; 95% CI: 1.18 - 4.87; p < 0.05), device (p < 0.05), skipping school to play games (OR = 3.97; 95% CI: 1.64 - 9.63; p < 0.05), no time limit for playing games (OR = 5.67; 95% CI: 2.31 - 13.94; p < 0.001), number of hours playing in a week (OR = 4.45; 95% CI: 1.14 - 17.41; p < 0.05). Conclusion: The rate of online gaming among students at Tam Nong High School is 89.23%. 16.38% of students who play online games have a screening score indicating a risk of online gaming disorder, according to the VN-IGD-17 scale. There is a statistically significant relationship between game genre, device, taking time off school to play games, no time limit for playing games, number of hours played per week with IGD.
Prevalence and associated factors of macrosomia among prenant women with gestational diabetes mellitus at Hai Phong Obstetrics and Gynecology Hospital
Objective: This study pursued two aims: (1) to determine the prevalence of macrosomia and (2) to identify factors associated with macrosomia among women with gestational diabetes mellitus (GDM) delivering at Hai Phong Obstetrics and Gynecology Hospital. Methods: Objective 1: A cross sectional descriptive study was conducted using convenience sampling of 3,164 women with GDM who delivered at the hospital from January 2021 to December 2024. Objective 2: A case–control study was carried out on 117 GDM women who delivered macrosomic infants (cases) and 234 GDM women who delivered non macrosomic infants (controls) during the same period and setting. Results: The prevalence of macrosomia among women with GDM was 3.95%. Univariate logistic regression analysis identified the following factors as increasing the risk of macrosomia: maternal age ≥ 30 years, parity ≥ 3, diet-only treatment, BMI, serum glucose, and HbA1c at the end of pregnancy. Intellectual labor (white-collar occupation) was found to be a protective factor. Multivariate logistic regression analysis revealed that only three variables were independently and significantly associated with macrosomia: Serum glucose at the end of pregnancy (OR = 1.33, 95% CI: 1.12 – 1.56, p = 0.001), BMI at the end of pregnancy (OR = 1.11, 95% CI: 1.03 – 1.20, p = 0.006), and HbA1c at the end of pregnancy (N=107, OR = 2.29, 95% CI: 1.34 – 3.92, p = 0.002). Conclusion: The prevalence of macrosomia among women with gestational diabetes mellitus (GDM) was 3.95%. Elevated serum glucose levels, higher body mass index (BMI), and increased hemoglobin A1c (HbA1c) levels at the end of pregnancy were independently associated with a higher risk of macrosomia in this population.
Epidemiological, clinical, and paraclinical characteristics of Dengue fever patients treated at the Department of Infectious Diseases, Viet Tiep Friendship Hospital in 2024
Objective: This descriptive study was conducted to identify the epidemiological, clinical, and laboratory characteristics of DHF patients treated at the Department of Tropical Diseases, Viet Tiep Friendship Hospital, in 2024. Methods: descriptive study. Results: Analysis of 350 patients revealed a nearly equal male-to-female ratio (51.4%/48.6%) with a mean age of 39.2 years. The most common clinical symptoms included high fever (66.9%), fatigue (64.9%), and headache (56.3%). Subcutaneous hemorrhage was observed in 40.3% of cases. Regarding laboratory findings, thrombocytopenia was a significant feature; 33.7% of patients presented with a platelet count between 10-50 G/L, typically reaching its lowest point on the sixth day of illness. Furthermore, elevated liver enzymes were common, with increased AST in 65.7% and increased ALT in 53.2% of cases, predominantly at a mild level (< 200 UI/L). These findings are crucial for the diagnosis and prognosis of patients with DHF.
Diagnostic value of Videonystagmography in vertigo patients at Hai Phong International Hospital
Objective: To describe the side effects experienced by women using Implanon NXT® and to examine associated factors. Methods: A cross-sectional descriptive study was conducted on 34 patients aged ³18 years presenting with dizziness . All patients underwent a full battery of Videonystagmography tests, including Saccade, Optokinetic, Dix - Hallpike and Caloric tests, between April and June 2025. Results: The mean age was 55,6 ± 14,0 years; 76,5% of patients were female. The most common etiologies were peripheral vestibular disorders (52,9%), followed by central (26,5%), mixed (14,7%), and unclassified causes (5,9%). Frequent symptoms included spinning vertigo (100%), nausea / vomiting (44,1%), and imbalance (41,2%). Saccade and Optokinetic tests demonstrated high sensitivity in detecting central causes of vertigo, while unilateral reduction Caloric response was more commonly associated with peripheral vestibular disorders (p<0,05). Conclusion: Videonystagmography is a reliable diagnostic tool in the evaluation of dizziness, especially for distinguishing between peripheral and central vestibular causes. Its application improves diagnostic accuracy, particularly in atypical presentations such as non – posterior canal BPPV of central vertigo with minimal neurological signs.
ide effect of subdermal contraceptive Implanon NXT® and associated factors with side effect
Objective: To describe the side effects experienced by women using Implanon NXT® and to examine associated factors. Methods: A cross-sectional study using retrospective data from women who inserted Implanon NXT® at Hai Phong University Hospital from to December 2023. Results: The most common menstrual change was irregular bleeding (38.33% within the first 6 months), which decreased to 20% in the following 6 months. Amenorrhea was reported in 26.67%, declining to 6.67% by the end of the second year. Other commonly reported side effects included weight changes (30%), vaginal dryness (35.83%), decreased libido (17.5%), acne (15%), breast tenderness (10.83%), headache (9.17%), and insomnia (4.17%). The implant removal rate due to side effects was 83.85%, with menorrhagia or prolonged bleeding accounting for 45.45% of removals. No statistically significant associations were found between user characteristics and the occurrence of side effects. Conclusion: The incidence of side effects following Implanon NXT® insertion is relatively high but tends to decrease over time. Menstrual disturbances were the most common adverse effect, with weight gain being the most frequent non-menstrual side effect. Menstrual disorders were the leading cause of implant removal. No significant correlations were identified between user characteristics and the occurrence of side effects.
Establishment of a technical protocol for EGRF mutation testing in patients with non-smal cell lung cancer (NSCLC)
Objective: Develop a standard technical procedure for testing to determine EGFR gene mutations from paraffin cast samples. Methods: Cross-sectional descriptive study, using Realtime-PCR technique in the process of determining gene mutations. Six paraffin cast tissue samples FFPE of patients diagnosed with lung cancer by histopathology and identified with EGFR gene mutations by molecular biology techniques were used to develop the procedure. Apply the procedure to detect EGFR gene mutations after being built on 109 paraffin cast samples FFPE of patients diagnosed with lung cancer by histopathology. Results: With 4-5 slices for each sample, the DNA concentration obtained ensures the quantity and input sample for the gene amplification reaction. When soaking in xylene twice in a row with each soaking time of 5 minutes under shaking and incubation conditions at 560C, the sample completely removed Paraffin, ensuring the purity of the input DNA of the gene amplification reaction. Of the 109 specimens tested, 38 were positive. Of these, 1 specimen was positive for both S768I and G719C; L858R had the highest positive rate of 31.58%, followed by ex19del at 28.95%; G719x and EGFR S768I both had a rate of 10.53%; EGFR ex20ins accounted for 7.89%, and finally EGFR T790M and EGFR L861Q accounted for 5.26%. Conclusion: Standardization and successful construction of the technical process for testing to determine EGFR gene mutations. The process gives higher test results, suitable for the facilities and equipment at the hospital. The rate of NSCLC patients identified to carry EGFR gene mutations of 34.86% is quite high.
Clinical and radiological characteristics of maxillary sinusitis caused by teeth at the Department of Dentistry and Maxillofacial Surgery, Hai Phong University of Medicine and Pharmacy Hospital
Objectives: Describe the clinical and paraclinical characteristics of odontogenic sinusitis, evaluate the treatment results of odontogenic sinusitis. Methods: Cross-sectional descriptive study on 30 patients diagnosed with odontogenic sinusitis. On cone beam computed tomography (CBCT), it is necessary to detect important signs that point to the cause of odontogenic sinusitis, such as ipsilateral maxillary sinus opacity and periapical inflammation, and periapical bone loss. Cone beam computed tomography is valuable in diagnosing maxillary sinus lesions and assessing in detail the causative tooth lesions, helping to plan combined sinus and tooth treatment. Results: showed that facial pain accounted for 90.6%, ipsilateral maxillary toothache accounted for 90.6%. Partial ipsilateral maxillary sinus opacity accounted for 62.5%, periapical inflammation accounted for 87.5%, periapical bone loss accounted for 84.4%. Thus, maxillary sinusitis due to teeth has two very valuable suggestive symptoms: facial pain and ipsilateral maxillary toothache. Treatment results: 100% of patients had stable maxillary sinus during dental treatment.
Comparison of Ultrasound-Guided Fine-Needle Aspiration Cytology and Postoperative Histopathology in Thyroid Surgery Patients at Viet Tiep Hospital, Hai Phong, 2024
Methods: A retrospective study was conducted on 120 patients with thyroid nodules who underwent ultrasound-guided fine-needle aspiration cytology (FNAC) at Viet Tiep Friendship Hospital in 2024. Results: the majority of patients were classified as TIRADS 3 (51.67%) and TIRADS 4 (44.17%), while TIRADS 5 accounted for only 4,16%. Ultrasound-guided FNAC of the thyroid demonstrated a sensitivity of 100%, specificity of 96.49%, accuracy of 98.33%, positive predictive value (PPV) of 96.92%, and negative predictive value (NPV) of 100%. All patients diagnosed cytologically as malignant were confirmed to have thyroid cancer on histopathology, and all cytologically benign cases were histologically non-malignant. Among six cases with cytology suspicious for malignancy, four were confirmed malignant and two were benign on histopathology.