Preliminary result on detecting reovirus in chickens farmed in Bac Giang province, 2022-2023

Abstract

This study aimed to identify reovirus infection in chickens raised in several districts in Bac Giang province. A total of 27 pooled samples including synovial fluid and tendons were collected from chickens with clinical signs of running-stuning syndorme, arthritis and tenosynovitis raised in Tan Yen, Yen The and Hiep Hoa district of Bac Giang province from October 2022 to January 2023. Results of polymerase chain reaction (PCR) showed that the number of positive samples for the avian reovirus (ARV) genome were 1/7 (14.29%) and 1/9 (11.11%) samples collected in Tan Yen and Yen The districts, respectively; whereas no sample was positive for the viral genome from chickens farmed in Hiep Hoa district. Analysis of σC gene sequences revealed that the field strains obtained in this study belonged to the cluster I and were genetically closed to the Chinese ARV strain. The finding of this study supplements  information on reovirus infection is a basis for eveloping diagnostic and disease prevention measures in chickens and further study.