Evaluation on bactericidal and inhibitory activity of herbal product against intestinal pathogenic bacteria in pig

Abstract

This study was conducted to test the bactericidal and inhibitory activity of herbal product on E. coli and Salmonella spp. isolated from pig. The research included 3 contents: Determining the moisture content of herbal powder, soaking the powder in absolute alcohol and water to extract and recover herbal extracts; determination of dry matter content and total polyphenols in herbal extracts; evaluate the activity and determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of herbal extracts. The studied results showed that moisture content of herbal powder was 3.35 ± 1.2%. The extractive efficacy for herbal extracts by water was higher 21.45% compared to extraction by alcohol. The average dry matter ratio of herbal extracts by alcohol and water was 921.6 mg/g (extracts) and 920 mg/g (extracts), respectively. The total polyphenols recorded from herbal extracts by water were 89.04 mg/g (extracts), (1gram of extracts containing 90% dry matter equivalent to 8.03 grams of herbal powder). The herbal extract product inhibited E. coli and Salmonella spp. MIC of both extracts (by water and by alcohol) on E. coli and Salmonella spp. ATCC strains and field strains was 0.1 mg/ml. The MBC
of herbal extracts by alcohol on E. coli was 0.4 g/ml, and by water on E.coli was 0.8 g/ml. The MBC of herbal extracts by alcohol and water for Salmonella spp. was 0.8 g/ml and 0.4 g/ml, respectively. The studied results indicate the bactericidal and inhibitory activity of herbal extract product and the potential
for supplementing herbal extract product in feed diet to improve the digestive health of pigs.