Research on creating antibodies (IgY) against tembusu virus causing egg laying reduction syndrome in ducks

Abstract

The objective of this study aimed to produce specific egg yolk antibodies (IgY) to tembusu virus from the immunized hens by the rute of injecting TMUV strains isolated from the field. Virus strain TMUV REP.V1.01.22 with a 50% lethal dose for duck embryos (ELD50 ) was 10-5.32 when it was completely inactivated by binary ethylenimine (BEI) 0.2%, at a temperature of 370C for 24 hours. Twenty-week old ISA brown breed hens were used to induce immunity and collect eggs that produced antibodies with high titers when injecting inactivated antigen: TMUV- REP.V1.01.22 with a dose of 100 ELD50 , repeated 3 times, each time 2 weeks apart, the hens gave the highest TMUV antibody titer (9.89 log2), lasting and stable. The average IgY antibody titer in egg yolks was collected from the immunized hens, after purification, reached 7.63 log2 . Egg yolk IgY antibodies against Tembusu virus open up opportunities in the prevention and control of Tembusu disease in ducks.