Isolation and antimicrobial susceptibility of Escherichia coli isolated from dairy cows in Gia Lam district, Ha Noi City

Abstract

This study was conducted to investigate hygiene practices and antimicrobial susceptibility of Escherichia coli (E. coli) isolated from the dairy cows in household farms in Gia Lam district, Ha Noi City. The rectal swab samples were collected from cows in 82 dairy cow household farms, and information on livestock and hygiene practices was collected through the questionnaire from May to September 2023. E. coli was isolated and tested for antimicrobial susceptibility to 12 antimicrobial agents, using disc diffusion method. The studied results showed that 100% of households used water only for cleaning the barns, and 42.68% of households applied waste treatment measures. In addition, farmers did not use sufficiently protective equipments during working, and a large number
of farmers treated the diseases for cows on their own farms. Out of 75 isolated E. coli strains, there were 18 isolates resistant to at least one antimicrobial (accounting for 24%), with 15 resistant pattens and 9 multidrug resistance strains were observed. The highest rate of E. coli strains resisted to ampicillin was 17.33%, followed by chloramphenicol (10.67%), tetracycline (10.67%), streptomycin (9.33%) and sulfamethoxazole/trimethoprim (8.0%).