Development of enterotoxin gene expression bacteria of enterotoxigenic Escherichia coli caused diarrheal disease in piglets

Abstract

The objective of this study was to create recombinant enterotoxin gene expression cells. The genetic fusion of enterotoxin gene was amplified and attached into 24a(+) and pET32a(+) vector and then transfored to BL21 successfully. PCR amplification and nucleotide sequence were used for determination of genetic fusion of enterotoxin gene in BL21 strain. The recombinant proteins were evaluated by SDS-PAGE and the immunoreactivity was characterized by Western blotting. The studied results showed that nucleotide sequences of genetic fusion of enterotoxin genes were consistent with nucleotide sequences of STa, STb and LT on GeneBank. The desired recombinant protein was expressed in LB broth with 1mM IPTG substance as inducer.