Result of identifying V. harveyi, V. vulnificus, V. parahaemolyticus, V. alginolyticus species in barramundi raising in Khanh Hoa province

Abstract

From 191 barramundi (Lates calcarifer) suspected with Vibriosis, we identified Vibrio spp. in 149 samples.
The results of species determination showed that among 175 isolates, the Vibrio harveyi accounted for the
highest prevalence (45.71% - 80/175), followed by V.vulnificus (29.71% - 52/175), V. parahaemolyticus
(12.57% - 22/175), V. alginolyticus (4.57% - 8/175), and 13/175 (7.43%) was not specified. The similarity
level of 16S rRNA gene sequences of two V. harveyi strains isolated in Khanh Hoa were 99.2% - 99.93% in
comparison with those of V. harveyi strain on GenBank and the reference strain ATCC 33843. The results
of identifying Vibrio species on barramundi raising in Khanh Hoa province are premise for further studies on
selecting representative strains to develop vaccines against Vibriosis on barramundi.