Cultivation of PIPEC continuous cell line for infection with African swine fever attenuated vaccine virus ASFV-G-Delta-I177L/delta LVR strain

Abstract

African swine fever is a highly dangerous and contagious disease, which has appeared in Viet
Nam since 2019 and caused heavy losses for the pig industry. A major challenge in the production
of African swine fever vaccines is that there is no a stable, suitable cell line for a vaccine candidate
virus to replicate on an industrial scale. ASFV-G-Delta I177L/Delta LVR attenuated candidate
vaccine virus strain generated by deleting the I177L and LVR gene has been investigated, this virus
strain has the ability to adapt on a very stable porcine-derived PIPEC cell line (Plum Island Porcine
Epithelial Cells). This study was conducted with the aim of standardizing the process of PIPEC cell
culture (PIPIC – trade name for export), experimental infection with ASFV-G-Delta I177L/Delta LVR attenuated vaccine strain leading to research on the production process of attenuated vaccines against African swine fever for the pig herd in Viet Nam