Optimising saponin steroid extraction from Costus pictus leaves using response surface methodology and investigating antibacterial properties of extracts obtained under optimised conditions

Abstract

The aim of the study was to optimise the extraction of steroid saponins from Costus pictus leaves and investigate the antibacterial activity of the obtained C. pictus leaf extract. The influence of major single factors, including ethanol concentration, ratio of raw materials to solvent, and extraction time, were investigated, and the use of the response surface methodology provided optimal extraction conditions...