Isolation and selection of lipase-producing bacteria from restaurant wastewater

Abstract

Domestic waste is a problem in big cities. Liquid wastes from food and drink services can be clumped to form a fatty layer on the water surface, causing environmental pollution and clogging the flow of the sewer system. This study aims to select bacterial strains capable of producing highly active lipase that can be used to deal with pollution from liquid waste containing lipids from restaurants. There are 50 strains of bacteria with lipase activity that have been isolated from water samples collected at restaurants in Ho Chi Minh city areas. Screening based on the diameter of the lipid degradation zone, 10 selected strains have a resolution ring of 9-11 mm. Quantitative results showed that strains GV-04 and BT-06 isolated from wastewater samples in Go Vap and Binh Thanh districts areas showed the highest lipase activity (GV-04 was 0.3250 UI/ml, BT-06 was 0.3158 UI/ml). The identification results showed that the two strains, GV-04 and BT-06, were Bacillus subtilis and Bacillus amyloliquefaciens, respectively.