Identification and biological characteristics of Bacillus altitudinis strain T1008 isolated from affected saline soils.

Abstract

Bacillus altitudinis T1008 strain was isolated from saline soil samples collected in Ben Tre province using Pikovskaya and Luria-Bertani medium containing sodium chloride 1% was determined and identified to level species. The results showed that the colonies were irregularly round, white to pale yellow; the cell was short rod-shaped, and motile. This strain was a gram-positive, spore-forming bacterium and resistant to sodium chloride 5% level; hydrolysed the inorganic phosphate compounds, synthesised indole acetic acid under in vitro conditions, and grew and developed well at temperature between 25-40oC. The result of the 16S ribosomal RNA sequence analysis indicated that this strain showed a similarity of 100% to Bacillus altitudinis species, Bacillus genus. This is the first report of B. altitudinis T1008 strain as a potential microbial inoculant for crop production in affected saline soils.