Identifying special EST-SSR regions of Panax vietnamensis Ha & Grushv based on Next- generation sequencing

Abstract

Panax vietnamensis var. vietnamensis Ha & Grushv is an endemic species with a narrow distribution. Because of having many benefits and high prices, there is an increase in faked Ngoc Linh ginseng. Most of the faked Ngoc Linh ginseng samples are other Panax species or subspecies distributed in Vietnam. Thus, it is very important to authenticate the Ngoc Linh ginseng samples. In this study, the author sequenced the transcriptomes genome of Panax vietnamensis var. vietnamensis based on the Next-generation sequencing method using Illumina HiSeq^TM 4000 system to detect the special SSR regions of this ginseng. A total of 23,741,783 raw reads were obtained and assembled with a total volume of 7.08 Gb. From these raw reads, 262,999 unigenes with an average length of 133 Mb were generated and 101 typical SSR regions of Ngoc Linh ginseng were successfully identified.