Generating a construct harboring the rpb1 promoter for enhancing recombinant protein expression in the medicinal mushroom Cordyceps militaris

Abstract

Cordyceps militaris is a valuable medicinal mushroom that contains numerous bioactive ingredients such as cordycepin, adenosine, pentostatin, polysaccharides, and carotenoids. However, the contents of these substances in wild-type strains of C. militaris are relatively low. Recently, the genome of C. militaris has been fully
sequenced, and genes involved in the biosynthesis of beneficial constituents have been identified...