Micropropagation in vitro Dendrobium wattii originated from apical meristem culture

Abstract

Studies on micropropagation of Dendrobium wattii were conducted in order to conserve and develop this precious orchid species. The results showed that PLBs were formed from apical meristem culture on the ½ MS medium supplemented with NAA within 42 days. PLB longitudinal thin cell layer culture was used as a technique for PLBs micropropagation on MS medium containing 1 mg/l NAA and 0.5 mg/l BA were optimal for PLB formation. PLBs multiplied with 13.15 PLBs/initial PLB increase when they were subcultured once 56 days on the MS medium supplemented with 2 g/l peptone, 20 g/l sucrose, 1mg/l NAA, 0.5 mg/l BA, 0.5 g/l activated coal (AC) and 8 g/l agar. PLBs converted into normal plants with well-developed shoots and roots on the ½ MS medium supplemented with 0.5 mg/l NAA, 0.5 mg/l BA, 10 g/l sucrose, 10% coconut water (v/v), 0.5g/l AC, and 8 g/l agar after about 90 days. No abnormal morphological changes were found in Dendrobium seedlings with using this propagation methods.

Keywords: Apical meristem, 6-Benzylaminopurine (BA), Dendrobium wattii, longitudinal thin cell layer, α-naphtaleneacetic acid (NAA), peptone, PLB.