Isolation and structural determination of the main compounds in the fraction of N-Butanol from Polyscias balfouriana (Andre) L.H. Bailey

Abstract

Leaves of Polyscias balfouriana plants grown in Can Tho were sampled, identified as the correct species of the study, then extracted and fractionated using the technique of exhaust extraction and liquid-liquid extraction, from which fractions were obtained. The n-butanol fractions were further isolated by chromatographic techniques and their chemical structures determined by nuclear magnetic resonance spectroscopy. The results isolated four saponin triterpen compounds including Ladyginosid A (PB01), 3-O-b-D-glucopyranosyl-(1→4)-b-D-glucuronopyranosyl oleanolic 28-O-b-D-glucopyranosyl ester  (PB02), acid 3-O-[β-D-glucopyranosyl-(1à4)]-b-D-(6-O-methyl) glucuronopyranosyloleanolic (PB03), 3-O-b-D-glucuronopyranosyloleanolic 28-O-β-D-glucopyranosyl ester (PB04). The structure was determined by one -dimensional 2D NMR nuclear magnetic resonance and compared with reference material. The above four compounds found in the leaves of Polyscias balfouriana provide basic data for further studies on their pharmacological effects.