Evaluation of the hydrolysis efficiency of shortfin scad (Decapterus russelli) using alcalase for the production of soluble protein hydrolysate rich in amino acids
Keywords:
, ,Abstract
The research has been conducted on the hydrolysis process of Indian scad (Decapterus russelli) protein using Alcalase enzyme under natural pH conditions and a water/material ratio of 1/1. The results show that in order to achieve optimal efficiency, it is necessary to use an enzyme-substrate ratio of 93.48 AU/kg protein, to maintain a temperature of 60ºC for 6 hours. Following this process, the highest hydrolysis degree achieved was 73.01%, with a nitrogen recovery efficiency of 75.30% and a total volatile basic nitrogen of 0.8 g/l in the obtained hydrolysate solution. The final product contains 13.45 g/l of amino acid nitrogen and 0.59 mg/100 g of histamine, demonstrating a significant utilisation for diverse applications across various fields.
