STUDY ON EXTRACTION OF CHLOROPHYLL FROM TO PRODUCE THE CHLOROPHYLL POWDER

Abstract

Chlorophyll was used as a dietary supplement that scavenging free radicals and helps prevent disadvantages for health. Some factors affecting the chlorophyll yield from lolot leaf (Piper sarmentosum) were studied, including the ratio of lolot leaf and ethanol and the extracting temperature. The response surface methodology (RSM) was used to maximize the simultaneous effect of ethanol concentration and extracting time. Then, a water-soluble chlorophyll powder was trial-produced and assessed for the chlorophyll content of product at the different temperatures of preservation. Immersion at 40^oC, the ratio of lolot leaf and ethanol of 1 and 15 was most effective for the extraction of chlorophyll. By RSM, the highest yield of chlorophyll was recorded at the ethanol concentration of 90% for 23.4 hours, yielding 34.8mg/g lolot leaf. Comparison with room temperature, preservation of the product at the cold temperature helped the chlorophyll content be more stable. The chlorophyll content of product reduced 17.5% after 6 months of preservation at 5^oC.