EFFECT OF CULTIVATION CONDITIONS ON MYCELIAL BIOMASS ACCUMULATION OF TERMITOMYCES CLYPEATUS

Abstract

The study was conducted to evaluate the effects of various culture conditions—including initial pH, shaking speed, temperature, inoculum ratio, and aeration rate—on the growth and biomass accumulation of Termitomyces clypeatus mycelia in submerged culture. The results showed that all three main factors (pH, shaking speed, and temperature) had statistically significant effects (p < 0.05) on the dry biomass yield. Among them, temperature had the greatest influence, followed by pH and shaking speed. The highest biomass yield (6.37g/L) was obtained at pH 4.5, 140rpm shaking speed, and 28°C. Response surface analysis using the Box-Behnken model predicted optimal conditions of pH 4.75, shaking speed of 131.3rpm, and temperature of 28.1°C, with a predicted biomass yield of 6.503g/L. Experimental validation under these optimal conditions resulted in an average dry biomass yield of 6.231 ± 0.213g/L, showing no statistically significant difference compared to the predicted value. In addition, the inoculum ratio and aeration rate also affected biomass accumulation. The combination of 10% (v/v) inoculum and an aeration rate of 0.4 v/v/m yielded 5.99g/L of biomass, which was higher than that of other tested conditions.