INDUCTION OF ATOPIC DERMATITIS-LIKE LESIONS BY TOPICAL APPLICATION OF 2,4-DINITROCHLOROBENZENE IN SWISS ALBINO MICE

Abstract

Atopic dermatitis (AD) is a common inflammatory skin disease for which there is lacking effective treatment. The development of different AD models will support studies of molecular pathology and provide a research tool for testing novel therapeutics. Our study aimed to induce the development of AD-like lesions by topical application of 2,4-Dinitrochlorobenzene on the dorsal skin of Swiss albino mice and characterize its manifestation. Subjects and methods: DNCB was applied topically on the dorsal skin of Swiss albino mice to sensitize and induce the development of AD-like lesions and symptoms. Pathological parameters including degrees of skin inflammation and damage, body weight, and the hypertrophy of secondary lymphoid organs were then measured. Results: the development of AD-like lesions with different degrees of AD symptoms was observed by DNCB treatment. Spleen hypertrophy and lymph node enlargement were also recorded. Conclusion: an induction of AD-like lesions could be achieved by topical application of DNCB in the dorsal skin of Swiss albino mice, thereby providing an additional approach for studying disease mechanisms in vivo.