ANTIOXIDANT AND ANTIINFLAMMATORY ACTIVITIES IN VITRO OF CORDYCEPS TAKAOMONTANA DL0038A EXTRACTS ISOLATED IN VIETNAM

Abstract

Cordyceps takaomontana was found on the LangBiang mountain – Da Lat, Lam Dong province, Vietnam at the height of 1650m above sea level. C. takaomontana belongs to the group of insect parasitic fungi used in traditional medicine in Asian countries for hundreds of years because of high value biological activities such as antioxidant, antiinflammatory, anticancer... In this study, extracts from C. takaomontana DL0038A fungi biomass and fruiting body were cultured in liquid – static media and investigated antioxidant activity by the methods of reducing power, free radicals scavenging such as DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2´azinobis (3-ethylbenzothiazonline-6-sulfonate); and antiinflammatory effect by the techniques of inhibiting xanthine oxydase (XO) and bovine serum albumine (BSA) denaturation. The results demonstrated that all seven fungal biomass extracts possessed antioxidant and antiinflammatory activities higher than the fruiting bodies. The ethyl acetate (EtOAc) extract from fungal biomass exhibited the strongest reducing power with the absorbance of 0.124 at the concentration of 1000µg/ml, and DPPH scavenging activity (IC₅₀ = 785.4 ± 15.689mg/ml); meanwhile the polysaccharides (IPS) extract from fungal biomass showed the highest ABTS scavenging capacity (IC₅₀ = 822.370 ± 6.210mg/ml). Moreover, the petroleum ether (PE) extract from fungal biomass inhibited the thermal denaturation of BSA with the IC₅₀ value of 139.790 ± 18.044mg/ml and the ethanol (EtOH) extract from fungal biomass showed the highest XO inhibitory effect with the IC₅₀ value of 236.564 ± 5.533µg/ml. All results showed that C. takaomontana DL0038A could be applied as potential bioactive materials in the production of funtional food ingredients.