STUDY ON THE ANTIOXIDANT ACTIVITIES OF SPHAGNETICOLA TRILOBATA EXTRACTS

Abstract

Sphagneticola trilobata (L.) Pruski is the scientific name for SAI DAT BA THUY (SDBT). The purpose of this study was to investigate the antioxidant activity of the crude extract and the class of fractions to which the potential antioxidants in the crude extract of SDBT belong. To do this, we extracted crude methanol extract and fractionated it with increasing polarity of n-hexane, chloroform, ethyl acetate, and water, respectively. The crude methanol extract had the EC50 value (DPPH) of 185.00 ± 4.31µg/ml, whereas the ethyl acetate extract had the lowest EC₅₀ values, indicating the strongest DPPH scavenging action (EC₅₀ values for n-hexane, chloroform, ethyl acetate, and aqueous were > 800.00µg/ml, 625.64 ± 43.05µg/ml, 19.67 ± 0.98µg/ml, and 478.17 ± 34,67µg/ml, respectively). Semi-polar compounds may possess antioxidant activity. Additionally, there appeared to be a strong correlation between the phenolic content of the extracts and their antioxidant activity, with the ethyl acetate fraction carrying the highest phenolic content exhibiting the strongest antioxidant activity.