DETERMINATION OF TRANSLATIONAL DIFFUSION COEFFICIENT OF A SINGLE GOLD NANOPARTICLE IN WATER + GLYCEROL MIXED

Abstract

In this paper, we present the study of the diffusion coefficient of a single gold nanoparticle in the water + glycerol complex medium by single molecule tracking method. The spherical gold nanoparticles are fabricated by Turkevich method with average size of 15 nm and dispersion in water. After fabrication, gold nanoparticles are placed in the water + glycerol mixture with different proportions. It is easy to observe and track nanoparticles of Brownian motion under dark-field optical microscopy, thereby determining the mean square displacement (MSD), then determining the diffusion coefficient D. The medium of water + 20%, 30%, 40%, 50%, 60%, 70% and 90% glycerol were investigated. The results show that the diffusion coefficient D of a single nanoparticle is inversely proportional to the amount of glycerol in the solution containing the grain. This result is important to the study of the dynamic of proteins in living cell membranes -one direction is of interesting.