PROPAGATION OF ME LINH ROSE BY PLANT TISSUE CULTURE

Abstract

Rose is considered as one of the most important cut flower species in the world and in Vietnam. In this study, stem segments of rose obtained from Me Linh (Ha Noi) were used as the materials for the micropropagation. The results showed that all of the treatments supplemented BAP were stimulated generating shoot and the concentration of 1.0 mg/L BAP was found as the best formula for the growth of regenerated shoots. The formula of MS with 1.0 mg/L BAP was highly recommended for the multiplication of rose nodal explants as the shoots number per explant were 3.80, the length of shoot  was 1.70 cm and the leaves number per shoot  reached 4.20. In the case of adding 0.2 mg/L activated charcoal, the length of newly regenerated shoots were found to be the highest one (approximately 2.70 cm) as compared with other formulas. Next, the medium ½ MSwith 0.50 mg/L NAA showed the efficiency for the in vitro root formation, with 67.50 roots per microshoot. Finally, in vitro plantlets were acclimatized by growing in TS 1 mixture substrate which exhibited the highest survival rate by approximately 75.5% after 2 weeks.