TESTING BIOACTIVE AND DETERMINATION OF JATRORRHIZINE CONTENT BY USING LC/MS METHOD IN THE STEM OF MAHONIA NEPAULENSIS DC. IN DAI TU, THAI NGUYEN

Abstract

Mahonia nepaulensis DC. is one of many valuable medicinal herbs in Vietnam. In this study, we have conducted a survey of substance layers, tested bioactive and determined of jatrorrhizine content in the stem of M. nepaulensis. The research methods used include extraction method to obtain the extract, chemical methods for surveying layers of the extracted liquid of the M. nepaulensis, bioactive test method and a standardized method for calibration line to determine jatrorrhizine content was used in this study. Phytochemical analysis showed the presence of alkaloids, steroids, coumarin and cardiac glycosides in the ethanol extract of the stem of this plant. The ethanol extract showed weak resistance to the Fusarium oxysporum (200 µg/mL), and n-butanol showed weak resistance to the Staphylococcus aureus (149.3 µg/mL). The content of jatrorrhirne in the M. nepaulensis is 0.682203%. The determination of the jatrorrhirne content in the stem of this species is an important significance in researches for the treatment of diabetes, psoriasis and alzheimer.