STUDY ON AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION OF GmDREB6 GENE IN VIETNAMESE SOYBEAN CULTIVAR DT22

Abstract

Transcription factor studies of the DREB subfamily have confirmed that GmDREB6 is related to salt tolerance in soybean plants. In this report, the GmDREB6 gene was used to genetically transform into 450 cotyledons of soybean cultivar DT22 through Agrobacterium tumefaciens carrying transgenic vector pBI121_GmDREB6. Of the samples infected with recombinant A. tumefaciens, 185 transformed samples were regenerated multiple-shoots and 583 shoots were produced. Selective results by kanamycin antibiotic in SIM and SEM medium obtained 109 shoots to switch to rooting medium and 53 plants were planted on the substrate and 12 plants growing normally under greenhouse conditions. Molecular analysis of 12 transgenic lines in the T0 generation had 8 lines positive for PCR, the transgenic efficiency at the PCR analysis stage was 1.78%.